"study on glutathione s-transferase inhibition assay by triclabendazole on fasciola spp. "
نویسندگان
چکیده
glutathione s-transferase (gst) represents the major class of detoxification enzymes from helminth parasites such as fasciola hepatica and f. gigantica and it is a candidate for chemotherapeutic and vaccine design. therefore, gst enzyme of fasciola spp. could be a target for evaluation of drugs such as triclabendazole (c14h9cl3n2os). for this purpose, gst enzymes were purified from fasciola spp. and sheep liver tissue by glutathione affinity chromatography using a wash-batch method and subsequently their sds-page pattern was detected. afterward, gst specific activity levels were assayed in the whole extract and purified solutions spectrophotometrically at 30°c with reduced glutathione (gsh) and 1-chloro-2, 4-dinitrobenzen (cdnb) substrate. finally, gst inhibition assay was investigated in the solutions by powder and bolus forms of triclabendazole. gst fraction as a 26 kda (mw) band was obtained on sodium dodecyl sulfate- polyacrylamide gel electrophoresis (sds-page). the level of gst specific activity in purified solutions was detected 18.14 µmol/min/mg proteins for fasciola hepatica, 35.04 for f. gigantica and 37.84 µmol/min/mg protein for liver tissue. comparison of the effect of powder and bolus of triclabendazole in solutions revealed inhibition concentration (ic50) 8.36 and 9.05 µg/ml for fasciola hepatica gsts and 7.20 and 10.80 for f. gigantica gsts and 8.65 and 9.70 µg/ml for liver tissue gsts, respectively. these findings suggest the possibility of selective inhibition of fasciola spp. gsts by triclabendazole in vitro and use of these results for understanding of its effect in vivo and qualification of manufacturing bolus form of drug in comparison with original powder.
منابع مشابه
"inhibition assay study of purified glutathione s-transferase from fasciola hepatica and sheep liver tissue by hexachlorophene"
glutathione s-transferases (gsts) are widespread in fasciola. hepatica parasite and sheep liver tissue. study of gsts inhibition assays in f. hepatica and sheep liver tissue are a priority of chemotherapeutic targets in parasitic liver diseases including human fascioliasis in iran. in this research, the whole extract of f. hepatica and sheep liver tissues were purified and eluted for sodium dod...
متن کاملstudy on glutathione s-transferase (gst) inhibition assay by triclabendazole. і: protoscoleces (hydatid cyst; echinococcus granulosus) and sheep liver tissue
hydatid disease is a term used to refer infection with the methacestode of echinococcus granulosus parasite in humans, and echinococcusis is restricted to infection with the adult stage in carnivores.glutathione s-transferase (gst) represents the major class of detoxification enzymes from helminth parasites such as echinococcus protoscoleces (psc) and it is candidate for chemotherapeutic and va...
متن کاملInhibition Assay Study of Purified Glutathione S-transferase from Fasciola Hepatica and Sheep Liver Tissue by Hexachlorophene
Glutathione S-transferases (GSTs) are widespread in Fasciola. hepatica parasite and sheep liver tissue. Study of GSTs inhibition assays in F. hepatica and sheep liver tissue are a priority of chemotherapeutic targets in parasitic liver diseases including human fascioliasis in Iran. In this research, the whole extract of F. hepatica and sheep liver tissues were purified and eluted for sodium dod...
متن کاملComparative assay of glutathione S-transferase (GSTs) activity of excretory-secretory materials and somatic extract of Fasciola spp parasites.
Fascioliasis is a worldwide parasitic disease in human and domestic animals. The causative agents of fascioliasis are Fasciola hepatica and Fasciola gigantica. In the recent years, fasciola resistance to drugs has been reported in the many of publications. Fasciola spp has detoxification system including GST enzyme which may be responsible for its resistance. Therefore , the aim of the study wa...
متن کاملDifferential Activities of Glutathione S-Transferase Isoenzymes in Strains of Fasciola Hepatica Susceptible and Resistant to Triclabendazole
Corresponding Author: V. Fernández Laboratorio de Biología Celular y Molecular. Centro de Investigación Veterinaria de Tandil, Argentina Email: [email protected] Abstract: Fasciolosis, a parasitic zoonosis of intrahepatic location, is caused by the trematode Fasciola hepatica. Its control is mainly based on the use of the anthelminthic Triclabendazole (TCBZ). The indiscriminate use of t...
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عنوان ژورنال:
acta medica iranicaجلد ۴۴، شماره ۶، صفحات ۳۷۱-۳۷۶
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